mouse anti rat cd49a mab  (Bio-Rad)

Journal: Immunity

Article Title: microRNA-142 is critical for the homeostasis and function of type-1 innate lymphoid cells

doi: 10.1016/j.immuni.2019.06.016

Figure Lengend Snippet: The CD3−NK1.1+NKp46+ compartment of Mir142+/+ (open green) and Mir142−/− (filled blue) mice was assessed for CD49a and CD49b by flow cytometry. (A) Representative flow plots of CD3−NK 1.1 +NKp46+ cells. (B) Total number of NK cells (NK, CD49a−CD49b+), ILC1-like (CD49a+CD49b+), and ILC1s (CD49a+CD49b−) within the indicated tissues. Summary from >3 independent experiments with > 10 mice per group. (C) Total numbers of NK, ILC1-like, and ILC1 cells within control (Ncr1-cre+ Mir142+/+) and ILC-specific miR-142-deficient (Ncr1-cre+ Mir142f/f) mice, cells gated on CD3−NK1.1+NKp46+ YFP+. (D) Experimental Schema. BM from control and Ncr1-cre+ Mir142f/f mice were transferred into irradiated recipients and spleens assessed 6 weeks later. (E) Summary data showing the percent of ILC1-like cells from CD3-NK1.1+NKp46+ CD45.1+ (WT) or YFP+ (Mir142f/f) within the indicated recipient mice. Data from 2-3 independent experiments, N=7-10 mice, compared using T-test or Mann-Whitney corrected for multiple comparisons, when appropriate. See also Figure S2.

Article Snippet: BV605 Hamster anti-rat/mouse CD49a mAb (Ha31/8) , BD Biosciences , Cat #740375.

Techniques: Flow Cytometry, Irradiation, MANN-WHITNEY

Journal: Immunity

Article Title: microRNA-142 is critical for the homeostasis and function of type-1 innate lymphoid cells

doi: 10.1016/j.immuni.2019.06.016

Figure Lengend Snippet: (A) Summary data of integrin αVβ3 and CD49a on CD3−NK1.1+NKp46+CD49b+ cells from the SP of Il15−/− or control mice. (B) Representative histogram showing integrin αV expression in the indicated tissues of Global and Ncr1-cre+ mice. (C) Summary showing percent integrin αV+ NK1.1+NKp46+ cells in the indicated tissues from Mir142−/− (left), Ncr1-cre+ Mir142f/f (YFP+, right), and controls. (D) Predicted miR-142-3p binding site in 3’UTR of Itgav (top). Luciferase reporter assay for Itgav 3’UTR (bottom). Data summarize 3 independent experiments and were compared using an ANOVA. (E) Mir142−/− mice were treated for 2 weeks with an αV inhibitor or vehicle. Summary data of total type-1 ILCs in the SP from 4 independent experiments, n=11 mice per group. (C, E) Data were compared using Student’s T test. See also Figure S3.

Article Snippet: BV605 Hamster anti-rat/mouse CD49a mAb (Ha31/8) , BD Biosciences , Cat #740375.

Techniques: Expressing, Binding Assay, Luciferase, Reporter Assay

Journal: Immunity

Article Title: microRNA-142 is critical for the homeostasis and function of type-1 innate lymphoid cells

doi: 10.1016/j.immuni.2019.06.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: BV605 Hamster anti-rat/mouse CD49a mAb (Ha31/8) , BD Biosciences , Cat #740375.

Techniques: Recombinant, Derivative Assay, Electron Microscopy, Cell Isolation, Flow Cytometry, Luciferase, Microarray, Control Assay, Software

Journal: Immunity

Article Title: microRNA-142 is critical for the homeostasis and function of type-1 innate lymphoid cells

doi: 10.1016/j.immuni.2019.06.016

Figure Lengend Snippet: The CD3−NK1.1+NKp46+ compartment of Mir142+/+ (open green) and Mir142−/− (filled blue) mice was assessed for CD49a and CD49b by flow cytometry. (A) Representative flow plots of CD3−NK 1.1 +NKp46+ cells. (B) Total number of NK cells (NK, CD49a−CD49b+), ILC1-like (CD49a+CD49b+), and ILC1s (CD49a+CD49b−) within the indicated tissues. Summary from >3 independent experiments with > 10 mice per group. (C) Total numbers of NK, ILC1-like, and ILC1 cells within control (Ncr1-cre+ Mir142+/+) and ILC-specific miR-142-deficient (Ncr1-cre+ Mir142f/f) mice, cells gated on CD3−NK1.1+NKp46+ YFP+. (D) Experimental Schema. BM from control and Ncr1-cre+ Mir142f/f mice were transferred into irradiated recipients and spleens assessed 6 weeks later. (E) Summary data showing the percent of ILC1-like cells from CD3-NK1.1+NKp46+ CD45.1+ (WT) or YFP+ (Mir142f/f) within the indicated recipient mice. Data from 2-3 independent experiments, N=7-10 mice, compared using T-test or Mann-Whitney corrected for multiple comparisons, when appropriate. See also Figure S2.

Article Snippet: PE anti-rat/mouse CD49a mAb (Ha31/8) , BD Biosciences , Cat #562115.

Techniques: Flow Cytometry, Irradiation, MANN-WHITNEY

Journal: Immunity

Article Title: microRNA-142 is critical for the homeostasis and function of type-1 innate lymphoid cells

doi: 10.1016/j.immuni.2019.06.016

Figure Lengend Snippet: (A) Summary data of integrin αVβ3 and CD49a on CD3−NK1.1+NKp46+CD49b+ cells from the SP of Il15−/− or control mice. (B) Representative histogram showing integrin αV expression in the indicated tissues of Global and Ncr1-cre+ mice. (C) Summary showing percent integrin αV+ NK1.1+NKp46+ cells in the indicated tissues from Mir142−/− (left), Ncr1-cre+ Mir142f/f (YFP+, right), and controls. (D) Predicted miR-142-3p binding site in 3’UTR of Itgav (top). Luciferase reporter assay for Itgav 3’UTR (bottom). Data summarize 3 independent experiments and were compared using an ANOVA. (E) Mir142−/− mice were treated for 2 weeks with an αV inhibitor or vehicle. Summary data of total type-1 ILCs in the SP from 4 independent experiments, n=11 mice per group. (C, E) Data were compared using Student’s T test. See also Figure S3.

Article Snippet: PE anti-rat/mouse CD49a mAb (Ha31/8) , BD Biosciences , Cat #562115.

Techniques: Expressing, Binding Assay, Luciferase, Reporter Assay

Journal: Immunity

Article Title: microRNA-142 is critical for the homeostasis and function of type-1 innate lymphoid cells

doi: 10.1016/j.immuni.2019.06.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: PE anti-rat/mouse CD49a mAb (Ha31/8) , BD Biosciences , Cat #562115.

Techniques: Recombinant, Derivative Assay, Electron Microscopy, Cell Isolation, Flow Cytometry, Luciferase, Microarray, Control Assay, Software